Kadeena Cox became the first Briton since 1988 to win a medal in two sports at the same Paralympics as she took cycling gold in Rio.
Cox, who took T38 100m athletics bronze on Friday, won the C4-5 time trial as Dame Sarah Storey finished fourth.
Her success came shortly after GB’s Hannah Cockroft retained her T34 100m title and 15-year-old team-mate Kare Adenegan took silver.
Britain have won 35 medals in Rio, including 15 golds.
Cox told Channel 4: “I can’t believe it. I’m over the moon. I have only been doing it a year and a half and being on top of the world is amazing.”
Earlier, Andy Lewis won GB’s first gold of the day with victory in the PT2 Para-triathlon.
Sabrina Fortune claimed women’s F20 shot put bronze, while swimmer Alice Tai took bronze in the women’s S10 100m backstroke.
Toby Gold won silver and compatriot Andy Small bronze in the men’s T33 100m as Britain secured eight medals on the third day.
