UNB, Dhaka :
Scientists at the icddr,b, in partnership with Incepta Pharmaceuticals, have developed a locally-made low-cost dipstick device, Cholkit, for rapid and effective diagnosis of cholera in Bangladesh and beyond.
Generating new hopes of better management of the disease, the rapid diagnostic test (RDT) has successfully met requirements and guidelines for such
tests, which are capable of detecting Vibrio cholerae from stools, said a icddr,b press release.
Cholkit is an immunochromatographic test device – a dipstick that is dipped into a tube with stool specimen and provides qualitative result (coloured band) readable by the naked eye within a maximum of 15 minutes’ time.
A field evaluation of Cholkit has recently been published in the scientific journal PLOS Neglected Tropical Diseases, said Dr Firdausi Qadri, a scientist in the Infectious Diseases Division at icddr,b who led the development of Cholkit.
“It showed that the sensitivity and specificity of Cholkit is similar to the commercially available RDTs, when used in field settings for detecting V. cholerae from stool specimens,” she said.
A total of 7,720 stool samples were tested during the evaluation where Cholkit has shown sensitivity of 76 percent and specificity of over 90 percent while other RDTs showed around 72 percent and 86.8 percent respectively.
The gold standard for detecting cholera is laboratory confirmation by stool culture, which is sensitive to several factors, including the quality of sampling, delays in shipment, laboratory equipment, skilled human resources, time (24 to 72 hours) and cost (6 to 8 US dollar/sample), the release added.
“From the public health perspective, the management of cholera outbreaks needs immediate detection as the pathogen has immense potential to spread and cause epidemics in a short period of time. Thus, the need for simple and easy to use RDTs which are quickly interpretable, require simple storage facilities, and are reasonably priced is a clear choice. It has the potential to be used at the primary health care level for cholera surveillance, for early outbreak detection and as a tool for an initial alert for monitoring of seasonal peaks in highly endemic areas and in peripheral health care facilities,” read the release.
Scientists at the icddr,b, in partnership with Incepta Pharmaceuticals, have developed a locally-made low-cost dipstick device, Cholkit, for rapid and effective diagnosis of cholera in Bangladesh and beyond.
Generating new hopes of better management of the disease, the rapid diagnostic test (RDT) has successfully met requirements and guidelines for such
tests, which are capable of detecting Vibrio cholerae from stools, said a icddr,b press release.
Cholkit is an immunochromatographic test device – a dipstick that is dipped into a tube with stool specimen and provides qualitative result (coloured band) readable by the naked eye within a maximum of 15 minutes’ time.
A field evaluation of Cholkit has recently been published in the scientific journal PLOS Neglected Tropical Diseases, said Dr Firdausi Qadri, a scientist in the Infectious Diseases Division at icddr,b who led the development of Cholkit.
“It showed that the sensitivity and specificity of Cholkit is similar to the commercially available RDTs, when used in field settings for detecting V. cholerae from stool specimens,” she said.
A total of 7,720 stool samples were tested during the evaluation where Cholkit has shown sensitivity of 76 percent and specificity of over 90 percent while other RDTs showed around 72 percent and 86.8 percent respectively.
The gold standard for detecting cholera is laboratory confirmation by stool culture, which is sensitive to several factors, including the quality of sampling, delays in shipment, laboratory equipment, skilled human resources, time (24 to 72 hours) and cost (6 to 8 US dollar/sample), the release added.
“From the public health perspective, the management of cholera outbreaks needs immediate detection as the pathogen has immense potential to spread and cause epidemics in a short period of time. Thus, the need for simple and easy to use RDTs which are quickly interpretable, require simple storage facilities, and are reasonably priced is a clear choice. It has the potential to be used at the primary health care level for cholera surveillance, for early outbreak detection and as a tool for an initial alert for monitoring of seasonal peaks in highly endemic areas and in peripheral health care facilities,” read the release.
